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addgene 43802  (Addgene inc)


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    Structured Review

    Addgene inc addgene 43802
    Addgene 43802, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cas9/p414-TEF1p-Cas9-CYC1t+(Plasmid+%2343802)/pmc12856427-1-10-10
    Average 93 stars, based on 126 article reviews
    addgene 43802 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: AcrIIA7 hijacks tracrRNA to block CRISPR-Cas system.
    Article Snippet: .. Additionally, expression constructs for Streptococcus pyogenes Cas9 (#62731), Neisseria meningitides Cas9 (#71474), Haemophilus parainfluenzae Cas9 (#121540), and Simonsiella muelleri Cas9 (#121541) were procured from Addgene. ..

    Construct:

    Article Title: AcrIIA7 hijacks tracrRNA to block CRISPR-Cas system.
    Article Snippet: .. Additionally, expression constructs for Streptococcus pyogenes Cas9 (#62731), Neisseria meningitides Cas9 (#71474), Haemophilus parainfluenzae Cas9 (#121540), and Simonsiella muelleri Cas9 (#121541) were procured from Addgene. ..

    Article Title: Cohesin and its regulation promote monopolar kinetochore orientation at meiosis I in Arabidopsis.
    Article Snippet: The complete assembly into the final destination vector pAGM4723 (Addgene #48015) was performed using a Bpi-based Golden Gate reaction. .. The construct incorporated the FAST-Red clone (Addgene #117499; pICSL11015) as a seed coat selection marker at position 1, followed by the Cas9 clone (Addgene #153224; pAGM51323), carrying an enhanced SpCas9 driven by the AtRPS5 promoter, at position 2. ..

    Article Title: Compositions and methods for targeted delivery of CRISPR-Cas effector polypeptides and transgenes
    Article Snippet: Plasmid Construction The Gag-pol expression plasmid psPax2 was a gift from Didier Trono (Addgene plasmid #12260). pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid #8454). .. Gag-Cas9 was constructed by amplifying Gag from psPax2 and Cas9 from pMJ920 (Addgene plasmid #42234). .. HIV-1 Env amino acid sequence was obtained from UniProt (P04578), human codon optimized (IDT), and ordered as a gBlock (IDT).

    Selection:

    Article Title: Cohesin and its regulation promote monopolar kinetochore orientation at meiosis I in Arabidopsis.
    Article Snippet: The complete assembly into the final destination vector pAGM4723 (Addgene #48015) was performed using a Bpi-based Golden Gate reaction. .. The construct incorporated the FAST-Red clone (Addgene #117499; pICSL11015) as a seed coat selection marker at position 1, followed by the Cas9 clone (Addgene #153224; pAGM51323), carrying an enhanced SpCas9 driven by the AtRPS5 promoter, at position 2. ..

    Marker:

    Article Title: Cohesin and its regulation promote monopolar kinetochore orientation at meiosis I in Arabidopsis.
    Article Snippet: The complete assembly into the final destination vector pAGM4723 (Addgene #48015) was performed using a Bpi-based Golden Gate reaction. .. The construct incorporated the FAST-Red clone (Addgene #117499; pICSL11015) as a seed coat selection marker at position 1, followed by the Cas9 clone (Addgene #153224; pAGM51323), carrying an enhanced SpCas9 driven by the AtRPS5 promoter, at position 2. ..

    Transduction:



    Plasmid Preparation:


    Article Title: Compositions and methods for targeted delivery of CRISPR-Cas effector polypeptides and transgenes
    Article Snippet: Plasmid Construction The Gag-pol expression plasmid psPax2 was a gift from Didier Trono (Addgene plasmid #12260). pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid #8454). .. Gag-Cas9 was constructed by amplifying Gag from psPax2 and Cas9 from pMJ920 (Addgene plasmid #42234). .. HIV-1 Env amino acid sequence was obtained from UniProt (P04578), human codon optimized (IDT), and ordered as a gBlock (IDT).


    CRISPR:

    Article Title: CRISPR screens in the context of immune selection identify CHD1 and MAP3K7 as mediators of cancer immunotherapy resistance.
    Article Snippet: .. CRISPR-Cas9 cell lines and tumoroids To generate Cas9-expressing cell lines, cells were transduced overnight with lentivirus containing Cas9 (pKLV2-EF1a-Cas9Bsd-W; Addgene #68343) plus polybrene (8 μg/mL; Thermo Fisher). ..



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